Insertion junction: CLIP2.046490_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046490
Chromosome:chromosome 14
Location:2747760
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre14.g626200 P4H7,PFH7,PHX18 Prolyl 4-hydroxylase 7; (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCTACCATCCTCGCAACCCTTAAATGCTACCAACGCGCAGAGGAGTGC
Internal bar code:TATCATTAGGGGAGGGGGGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:438
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCCAAACCCATACCGTAA
Suggested primer 2:GCTAGGATGGACTTGTCCGG