Insertion junction: CLIP2.046511_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.046511
Chromosome:chromosome 4
Location:2261274
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACAAGACTCCTTAAGTACCAGTCTCTAGTATTGTGACATAGAACGATC
Internal bar code:TAAAGGTAGCTATGTAGCTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1247
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTACCAGTTCGCCGAGGATC
Suggested primer 2:ACAACCGCCTTACCATCGTT