| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046529 |
| Chromosome: | chromosome 16 |
| Location: | 1160127 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650350 | (1 of 1) PF03457 - Helicase associated domain (HA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTGTCCGCCGACCTTGCCGCAGTCACCGGCCGACTGCTGGACATGA |
| Internal bar code: | TTTTCATTAAGGGGGACAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1814 |
| LEAP-Seq percent confirming: | 95.9184 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCCTTCCCTCCCTCCCTC |
| Suggested primer 2: | GGATGCTTACATGCGTGCTG |