Insertion junction: CLIP2.046531_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.046531
Chromosome:chromosome 9
Location:528037
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre18.g748597 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGAGTGTCTCTCCTCACCATGTCCATCACTCAAGATAAATGGGGTCGCG
Internal bar code:TGTGTGTGCTTCGTGCATAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2526
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGATACCAGCCCAAACCA
Suggested primer 2:GCCAGTGGCTATACATCCCC