Insertion junction: CLIP2.046532_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.046532
Chromosome:chromosome 2
Location:1016669
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g079926 PHC64 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACCCGAGTCGTGCTGCCCAGGTGAGATGTCGGGCTGAGGTGAAACCT
Internal bar code:GTTCTTCCGTTTACTTCGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3706
LEAP-Seq percent confirming:44.2529
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:97
LEAP-Seq n unique pos:174

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCTGCAAGCCTGTCATA
Suggested primer 2:CAGCTCCAAGTTTGCAACCC