| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.046536 |
| Chromosome: | chromosome 17 |
| Location: | 2444614 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715000 | HSP33 | Heat shock protein 33; (1 of 1) K04083 - molecular chaperone Hsp33 (hslO) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCGGGTGCTGGGGATGCGCAAGGCTGCAAACACCAACCATGCCGTGC |
| Internal bar code: | CATTTCTTCAATCTCCACCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 134 |
| LEAP-Seq percent confirming: | 5.26316 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | TCAGGCGTTAAGGGTCACAC |