| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.046540 |
| Chromosome: | chromosome 5 |
| Location: | 1530007 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234662 | (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGAGTAGTAGGCCTGTGGGGACGGTGCGTGGGTTGAGGACGCGACGTG |
| Internal bar code: | TTATTCGGGTCCCAAGTTTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTTATCCACCGGTGCGTT |
| Suggested primer 2: | CCTATGCCACGTCTCGAGTC |