Insertion junction: CLIP2.046556_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.046556
Chromosome:chromosome 7
Location:942010
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g319100 TEF18 Glutathione S-transferase-related protein; (1 of 1) 5.3.99.3 - Prostaglandin-E synthase / Prostaglandin-H(2) E-isomerase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGTCGCGTCTGTGCCCACTCCCTAGTAGGGATGTGCCACATGCCTGCC
Internal bar code:CGCCCCCAGTCCCAACTCGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:436
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTCTCCTCCATCTCCACA
Suggested primer 2:GTCACAATCCGCTCACTCCA