| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.046574 |
| Chromosome: | chromosome 9 |
| Location: | 5452338 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g801071 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCGCGGCCGTGCATACCGCCGCGGTGATCCAAGCCTCATCCCAAACCG |
| Internal bar code: | TTTTTAATTAAAATACACTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2437 |
| LEAP-Seq percent confirming: | 73.6842 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGCACACACATGACACG |
| Suggested primer 2: | GCAATAAAGGTAGCCGCAGC |