| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046578 |
| Chromosome: | chromosome 2 |
| Location: | 7210001 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800259 | CDS | ||
| Cre09.g390801 | (1 of 1) PTHR11918//PTHR11918:SF77 - RADICAL SAM PROTEINS // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTTACGCTCCCCACGCCGCCATTCATGTCCACGCTACCTTTATTACG |
| Internal bar code: | GTGTCGGTAAGAACCCGGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 522 |
| LEAP-Seq percent confirming: | 25.3968 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGTCCGTGAACAGATG |
| Suggested primer 2: | GGCCTCTTGCTAACCCACAT |