| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046602 |
| Chromosome: | chromosome 16 |
| Location: | 2039234 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656950 | (1 of 1) PTHR13140:SF300 - PROTEIN TAG-278-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGCGCGAACGCTGGCCTGCTGCTTGGCAACCTCCGCCTCCGCGGCGT |
| Internal bar code: | TTTTTTGCGGTTTTCTAGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2516 |
| LEAP-Seq percent confirming: | 7.27273 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 153 |
| LEAP-Seq n unique pos: | 165 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGAGCAGAAGTCCACAGC |
| Suggested primer 2: | AATCGGCTGTGTACTCCTGC |