| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.046606 |
| Chromosome: | chromosome 13 |
| Location: | 1131264 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569500 | (1 of 3) PTHR10696 - GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED; Similar to conserved hypothetical protein in bacteria | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGGCCGTCAGCTGCTGCTGGCGGCTGTAGCGCTGCACGGGCACGCCG |
| Internal bar code: | GTAGAGGACTTTGGGAGTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3835 |
| LEAP-Seq percent confirming: | 80.3571 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGCTGCATTCGACTAGAT |
| Suggested primer 2: | GGAGCACGAAGAGACAACCA |