| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.046608 |
| Chromosome: | chromosome 3 |
| Location: | 4149048 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172900 | (1 of 2) PF11371 - Protein of unknown function (DUF3172) (DUF3172) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCACAAAGACCTGTGGGAGCGCAGACGGGCCAAAGGTTGCTGTCAAG |
| Internal bar code: | TTGTTGGCTTGCATAATGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2272 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCCCTAGCACTCACAAA |
| Suggested primer 2: | CAGTGCAGGACTTTTGTGCC |