Insertion junction: CLIP2.046612_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.046612
Chromosome:chromosome 10
Location:1263033
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g426700 CYP739A4,CYP28 (1 of 1) 1.14.13.109 - Abieta-7,13-dien-18-ol hydroxylase / CYP720B1; Cytochrome P450, CYP197 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAACATCACATCAAACCTGCGCGCGATACTAGGCACGGAATCTGGTTGC
Internal bar code:TAAGCTGGCTGACGATGTTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1723
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:84
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTGCAGGAGGAGATATT
Suggested primer 2:CATCAGGTGTGGGTGGTACC