| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.046615 |
| Chromosome: | chromosome 16 |
| Location: | 2653025 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661450 | HTV3 | (1 of 35) K11253 - histone H3 (H3); Histone H3 variant | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGACCTGCATGCACATGCCAGCAGTTTTGGTATGTTGGATAATGTGT |
| Internal bar code: | TTATGGTTTTCCTTTAGGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1766 |
| LEAP-Seq percent confirming: | 28.3784 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGAAGCCAGTGAGTGTGA |
| Suggested primer 2: | GCGCCAGTTGCATATCCTTG |