| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046615 |
| Chromosome: | chromosome 16 |
| Location: | 2653029 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661450 | HTV3 | (1 of 35) K11253 - histone H3 (H3); Histone H3 variant | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAACCAGATGCTGCGCGAGCCCTTCCGTTGGACTGCGGAGGCACTAC |
| Internal bar code: | GCGTAGCAGTGGAGCACGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4902 |
| LEAP-Seq percent confirming: | 83.4862 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCCAGTTGCATATCCTTG |
| Suggested primer 2: | GTCGAAGCCAGTGAGTGTGA |