| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.046617 |
| Chromosome: | chromosome 3 |
| Location: | 6443709 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193250 | (1 of 1) 2.7.1.160 - 2'-phosphotransferase / Yeast 2'-phosphotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGAGGCCACGGGTGGCGGAAGCTATTTGTGCACGGGGTAGAGCAGC |
| Internal bar code: | GTCTTTCCATGGGCTAGTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4925 |
| LEAP-Seq percent confirming: | 88.3721 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAATTGGTAGAACGCTCG |
| Suggested primer 2: | CACCTCGGCCCACTTGTTAT |