Insertion junction: CLIP2.046640_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.046640
Chromosome:chromosome 3
Location:2972523
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g163150 (1 of 2) K09955 - hypothetical protein (K09955) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCTTTACTGCACCGTACTAAGGCAGTCCGGCACCCCACTGCCGCCCTG
Internal bar code:TAAGTGCGTTGCATCATGTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1451
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCGGGTCTATCTCAACGC
Suggested primer 2:GTGACGTGTGTTGTGTCGTG