| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.046647 |
| Chromosome: | chromosome 12 |
| Location: | 5149400 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526700 | RBL9 | Rhomboid-like protease; (1 of 11) 3.4.21.105 - Rhomboid protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGTCTTCAAACACGCACACATGCCCCGAGCCTCCCCTCTCGGCCACG |
| Internal bar code: | TGTAGCATTGGGGAATTGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3243 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 120 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 120 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGCTGACGGAAATCGCG |
| Suggested primer 2: | GTTGTGCGTGTGTGTCTTCC |