| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046651 |
| Chromosome: | chromosome 3 |
| Location: | 5218139 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| lncRNA_TCONS_00234913 |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCCTGCCGTTAAGCTGGGCTGACTCGAGCTGCCGCCGCGCTGGTGCT |
| Internal bar code: | AGGATATTGAAAATGGTTGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1900 |
| LEAP-Seq percent confirming: | 82.8125 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAATCAGTGCAGGTAGGT |
| Suggested primer 2: | CTGGGTCAAGACATTCCCCC |