| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.046655 |
| Chromosome: | chromosome 2 |
| Location: | 4944898 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110200 | THO1 | Oligoendopeptidase; (1 of 3) 3.4.24.59 - Mitochondrial intermediate peptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTCAGATCCTCACGCTCAGCCAGCAGCATCGACCAAAGTATTATGTA |
| Internal bar code: | ATTATTGTTGACGTTAAAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1940 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCGCAAATACCAGCTCCT |
| Suggested primer 2: | TGGGGCGGCATTGTCTATTT |