| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046670 |
| Chromosome: | chromosome 8 |
| Location: | 2099085 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACTGCGCAATGATTCCGCACCCCGCACCCCAGACCCCGCATCCCGCA |
| Internal bar code: | TTCTCATTAATTTAGGGGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 162 |
| LEAP-Seq percent confirming: | 23.0769 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGAAGTGTGCCGCTTTTC |
| Suggested primer 2: | GAACCATGCCACGAAGAGGA |