Insertion junction: CLIP2.046691_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046691
Chromosome:chromosome 16
Location:502059
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g692902 AHI1 Abelson-helper integration site 1; (1 of 3) PTHR22847:SF361 - JOUBERIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTTCACGCCACCGGCGCCGGTGCCGGCGCGCGGACGCAGGGTTGCGG
Internal bar code:ACGGTTGTTCAATAGACGATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:884
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGGTGTAGGTGTGTGGAG
Suggested primer 2:CACGTGGCGTCATTCATGTC