| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046725 |
| Chromosome: | chromosome 1 |
| Location: | 5526657 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039350 | NCR2 | Cytochrome P450 reductase, possibly CYP505 family; (1 of 2) 1.6.2.4 - NADPH--hemoprotein reductase / TPNH-cytochrome c reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGCCAAAGTGAGGAGCGGTACAGACTGGGTGTACCCGCAGCAGGCGT |
| Internal bar code: | GACTTCTTATTAATGTACCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5458 |
| LEAP-Seq percent confirming: | 92.7536 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGAAGCTGGTGGTGATGT |
| Suggested primer 2: | CCTCTAGGTCAAAGCCCAGC |