| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.046753 |
| Chromosome: | chromosome 3 |
| Location: | 6395335 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192600 | THK1 | (1 of 1) 2.7.1.21 - Thymidine kinase; Thymidine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTACTGCTGCCAGCTAGGCTCCGACACGGTAACTTAATCCGCAATCC |
| Internal bar code: | CTATTGAGGAGGTCGGAGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3487 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGAGAGGAACAGAAGTGC |
| Suggested primer 2: | GCACATTGGGAAAGCAAGCA |