| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046763 |
| Chromosome: | chromosome 9 |
| Location: | 151340 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386732 | (1 of 1) 2.4.1.57 - Phosphatidylinositol alpha-mannosyltransferase / Phosphatidyl-myo-inositol alpha-mannosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCTCACGCAGCAGCAGCAGCTGGCGGGGCTGCGGCCGCCCGCCTGGCT |
| Internal bar code: | TGTGCGCAATAATTGAATTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1346 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCTGTTGCTTCTGAGGC |
| Suggested primer 2: | GGCTATGTCGTACGGGTCTG |