| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.046769 |
| Chromosome: | chromosome 6 |
| Location: | 2013430 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265300 | CSS2 | Chlamydomonas specific family S protein; (1 of 7) IPR011048 - Cytochrome cd1-nitrite reductase-like, haem d1 domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGTAGGTGCCGCGGGGTTGAGTTGATCAGACACAGGCACGTGGCATC |
| Internal bar code: | GCTGTAGCTTAATAGTGTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1327 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACACTGGCGCTTGTTTGC |
| Suggested primer 2: | GAAGCCAAAAGGCAGGAAGC |