| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.046780 |
| Chromosome: | chromosome 1 |
| Location: | 6048201 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042700 | (1 of 140) IPR002048 - EF-hand domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCCATGTTTGGTCTCACGTGGGCGGTGGTGGCAGCGTTGAAGGACA |
| Internal bar code: | GTCGTCGCGTTCAGACTGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5430 |
| LEAP-Seq percent confirming: | 81.6667 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 120 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAATGTGCGGCTTGTTGA |
| Suggested primer 2: | CTTTCAGGCTTCAACACGCC |