Insertion junction: CLIP2.046785_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.046785
Chromosome:chromosome 12
Location:4657984
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g522300 (1 of 1) IPR001611//IPR002048//IPR003590//IPR008907//IPR011992 - Leucine-rich repeat // EF-hand domain // Leucine-rich repeat, ribonuclease inhibitor subtype // P25-alpha // EF-hand domain pair intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACATGCCCCGTCCCACGGGCCCTTCCCCACAGCACCACCACCCCCGGCA
Internal bar code:TGGCATCAAGCTCACCAGTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:76
LEAP-Seq percent confirming:7.14286
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AACAAGAACACCACCCTGCA
Suggested primer 2:GAGGTGGTTGTCGTCGTTCT