| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.046804 |
| Chromosome: | chromosome 16 |
| Location: | 5983258 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678600 | (1 of 3) PTHR20961:SF0 - EGF DOMAIN-SPECIFIC O-LINKED N-ACETYLGLUCOSAMINE TRANSFERASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCCGCAGTGTCAGCATGGGTCCCAAGCCGCATTCAATCTCCATTTAC |
| Internal bar code: | TAATATTTTACAAGATATTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1490 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAATCGCGTTCGTTCAGG |
| Suggested primer 2: | CGTCTCTCTACGAGCAGCTG |