| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.046809 |
| Chromosome: | chromosome 17 |
| Location: | 5969397 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742350 | (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGGAAACGGCAGAAGATGCAGCAGAAGCAGCAGCGGCGGCGGCGGTT |
| Internal bar code: | AGTAAATCATGAAACGCGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3269 |
| LEAP-Seq percent confirming: | 98.2759 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGAATACGGGAGGCCA |
| Suggested primer 2: | CTGTTCTCCGGCTCTTTGGT |