| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.046810 |
| Chromosome: | chromosome 1 |
| Location: | 5592453 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039850 | CPK5,CGL48 | (1 of 1) PF00294//PF03641 - pfkB family carbohydrate kinase (PfkB) // Possible lysine decarboxylase (Lysine_decarbox); conserved protein related to lysine decarboxylase domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCCAGCGCCCGCCCAACGTTGCTGGCGGACCCGCCAGCCTTGGAGTG |
| Internal bar code: | TTGCCAACCTATGTGCGAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAGCACACTACTTGGAG |
| Suggested primer 2: | ACAACGCCTCTGAGAACTGG |