| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.046813 |
| Chromosome: | chromosome 3 |
| Location: | 4715442 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177500 | (1 of 2) K17279 - receptor expression-enhancing protein 5/6 (REEP5_6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTGGAAGTAAAGGTGCAGCCAGGGTGCTGCGAGCGGCCGGCGCAACG |
| Internal bar code: | CTGTGACCTCAAATCCGTGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2242 |
| LEAP-Seq percent confirming: | 56.5217 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTCTGCTCATCCTACCT |
| Suggested primer 2: | CCTTCTATGCGTGCTAGCGA |