Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.046832 |
Chromosome: | chromosome 12 |
Location: | 6185478 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g535800 | (1 of 1) PF13191//PF13424 - AAA ATPase domain (AAA_16) // Tetratricopeptide repeat (TPR_12) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATGCAATAGAGCGAGGGGGCAGTAGGGACGCGGGGGGAGGGGTTTGG |
Internal bar code: | GGATTATGGTTGTTAGCGTGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 965 |
LEAP-Seq percent confirming: | 26.6667 |
LEAP-Seq n confirming: | 16 |
LEAP-Seq n nonconfirming: | 44 |
LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCCTACTTAGCCGTGCCTG |
Suggested primer 2: | GGCGACTTGGACACACAAAC |