Insertion junction: CLIP2.046840_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046840
Chromosome:chromosome 2
Location:5797079
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g117000 CYG21 (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCGTGGGCTTCACCAACATGTGCAAACAAGTGTCGCCGCTGGAGGTCA
Internal bar code:TTTAGACCGTGGATATCAAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2520
LEAP-Seq percent confirming:27.2727
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCAGGGCAGTGTTGGATG
Suggested primer 2:TCATCCTGGCATGACCCAAC