| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046848 |
| Chromosome: | chromosome 11 |
| Location: | 1327068 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467705 | (1 of 3) K02154 - V-type H+-transporting ATPase subunit a (ATPeV0A, ATP6N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACAGCCGGGCCGCATGCTCCAACACCACCTGTTTGTGTGTGAGAGATT |
| Internal bar code: | TCAGAGAGCGGTCGAATCAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1641 |
| LEAP-Seq percent confirming: | 99.2701 |
| LEAP-Seq n confirming: | 136 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 137 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGAACTGTCGTGGTCGA |
| Suggested primer 2: | AAAAGCCGTGGGAGAGATGG |