| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.046860 |
| Chromosome: | chromosome 7 |
| Location: | 2939568 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332786 | PSL2 | Signal peptide peptidase, eukaryotic-type; (1 of 1) K09597 - signal peptide peptidase-like 2B [EC:3.4.23.-] (SPPL2B) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGACACGCAAGTGTGAGGGGCCCCCGCCCTCACCAGCCAGTGCTCCCG |
| Internal bar code: | GTTCCGAGCGCCCCAGGATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1463 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTGTCACTGCACACAC |
| Suggested primer 2: | GCGCAAATCGGACGTCTAAC |