| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.046865 |
| Chromosome: | chromosome 12 |
| Location: | 699214 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492900 | (1 of 3) PF01661 - Macro domain (Macro) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATACCTGTTTCTGATGCCGGTGATGGGGCTGCGGGAAGTTGGAAAGG |
| Internal bar code: | AATTGGGGTGCCCGCTAGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4644 |
| LEAP-Seq percent confirming: | 90.6667 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCGCAACACCCTGATGTA |
| Suggested primer 2: | CACAGTGCGGATTTGGCAAA |