| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046939 |
| Chromosome: | chromosome 3 |
| Location: | 6018512 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189700 | (1 of 2) K07735 - putative transcriptional regulator (algH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACTCCATCGCCAGTTGCCCGGGCGCCCAGCCAGCGTATGCGCTGAAC |
| Internal bar code: | ATTATATTTTCTTATGTAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3485 |
| LEAP-Seq percent confirming: | 77.2727 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTTCTTGTGCGTTGACG |
| Suggested primer 2: | CAGGTTGAGCCAAGGAGGAG |