| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.046951 |
| Chromosome: | chromosome 1 |
| Location: | 5736592 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041100 | HLM3 | (1 of 1) PTHR12197:SF75 - HISTONE-LYSINE N-METHYLTRANSFERASE ATXR2; Histone-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGATTGTAAAAGGTATGAGGCCGCAATGTGAGCCGCGGCCCTCCCGA |
| Internal bar code: | GCTATGATGGTTGTTATCACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2517 |
| LEAP-Seq percent confirming: | 97.8723 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGAATCCAGCCATGCGTG |
| Suggested primer 2: | GGCCCTCCCCTTGTCTTAAC |