| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.046976 |
| Chromosome: | chromosome 13 |
| Location: | 2795119 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582650 | MAPK1,LF4 | (1 of 1) K08830 - renal tumor antigen [EC:2.7.11.22] (RAGE, MOK); Mitogen-activated protein kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCAGGTGGTACAGCCTTGCGCCCCTTGTCGCCCGCCCGTTTGCCCCT |
| Internal bar code: | TGGCGCATGCACGGCGGTAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGGGTCTTTCGTTACGT |
| Suggested primer 2: | CCCGGCTCGTAGTATGTGTT |