Insertion junction: CLIP2.046979_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.046979
Chromosome:chromosome 14
Location:1030112
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g614100 GTR26,DGL1 Dolichyl-diphosphooligosaccharide-protein glycosyltransferase; (1 of 1) K12670 - oligosaccharyltransferase complex subunit beta (WBP1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTTAAATGGCTCTACTCACAGTCTTCGAACTACTTCGCCTCAACCCCC
Internal bar code:GTAGAATGCGCTGACTGGGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1461
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGTCCTGTGTGTTGAAAC
Suggested primer 2:TCCATGCCTCGCTCTGTTTT