| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.046988 |
| Chromosome: | chromosome 9 |
| Location: | 1828488 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397850 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAAACTAAAACAGATAGCGCCAACCTTTTGCGCCTCGCGCTTTCGCG |
| Internal bar code: | GGACCTCATCGTGGCCTCGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5658 |
| LEAP-Seq percent confirming: | 91.2281 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 114 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCCTACTTGGTTCACCTT |
| Suggested primer 2: | TACAAGGTGGTGAGCACGAC |