| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046994 |
| Chromosome: | chromosome 9 |
| Location: | 4463422 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400738 | (1 of 1) PTHR23012//PTHR23012:SF96 - MEMBRANE ASSOCIATED RING FINGER // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTTGGTGGCCCTGGTATCCGGCCAGCTCCGAATCCCAGCCAGTCCCCC |
| Internal bar code: | TGGAGCGTGTGATTCAATGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2541 |
| LEAP-Seq percent confirming: | 59.7701 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCGCTAAACCCCTGTAAG |
| Suggested primer 2: | GAGTCTGGATGCAGTGGCTT |