| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047005 |
| Chromosome: | plastome |
| Location: | 202705 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802336 | orf854 | Wendy transposon | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTTCGTCCAGGAGTGGGTGACGCCCTCAGTAAGATGGAAGATTCTCT |
| Internal bar code: | TTCGTATAGTGAAGTTCCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTCTCCTTACGGGCAAG |
| Suggested primer 2: | TTGACGCCTAAGCCGGAATT |