| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047015 |
| Chromosome: | chromosome 10 |
| Location: | 5620453 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458600 | FAP245 | Flagellar Associated Protein 245 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACACGTTAAGAAAGTCCCACGACCCGGGTCGCAACTAGGTCCAATTTG |
| Internal bar code: | TTAAGCATATGATTTTTTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2059 |
| LEAP-Seq percent confirming: | 18.1818 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCTGAAACAGCCGTGTA |
| Suggested primer 2: | TGAGTTCCGAGTTTGCCCTC |