Insertion junction: CLIP2.047020_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.047020
Chromosome:chromosome 5
Location:1493687
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g234654 (1 of 1) IPR002110//IPR003613//IPR013083//IPR020683 - Ankyrin repeat // U box domain // Zinc finger, RING/FYVE/PHD-type // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATACACACACGCACGCTGCATCGCATACACTGTCTTTGCGCAACGTTCT
Internal bar code:TGTGCTTACTTTATTGATAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1024
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGGATCCATGCTTATCGA
Suggested primer 2:GATAAGCGACACACAACGGC