Insertion junction: CLIP2.047034_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.047034
Chromosome:chromosome 8
Location:4240488
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre08.g384200 (1 of 1) IPR000104//IPR003191//IPR009148//IPR015894//IPR027417//IPR030386 - Antifreeze protein, type I // Guanylate-binding protein, C-terminal // Streptococcal non-M secreted SibA // Guanylate-binding protein, N-terminal // P-loop containing nucleoside triphosphate hydrolase // GB1/RHD3-type guanine nucleotide-binding (G) domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACCCACATCGCACGCACACTCCCCGCTCACTCGCTCGCCCCCGCACC
Internal bar code:GACGTATTTCAGAGAAGTAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:728
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTAAACAACGTTTGCCCA
Suggested primer 2:GGGAGGGGAGGTTGTAGCTA