| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.047046 |
| Chromosome: | chromosome 8 |
| Location: | 4188516 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCTCAAGGAGCAGGTGAGTTGTTCTATCTGGCAGCTGGCAGGCATGT |
| Internal bar code: | CATGTGGGGGGCTTCTGGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2361 |
| LEAP-Seq percent confirming: | 37.931 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATGATCGACCCACCCAG |
| Suggested primer 2: | CAAAGCCCGGTTGGTCAAAG |