| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.047053 |
| Chromosome: | chromosome 10 |
| Location: | 6515873 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGTGCGAAACCCGGAGCGCACAGGGGCACTCACCTGGAGCGGGGCCA |
| Internal bar code: | TGAGGTGCTAACATTATACCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1416 |
| LEAP-Seq percent confirming: | 45.4545 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTAGTGTTGCAACGGTA |
| Suggested primer 2: | CAACAGCTCCTCCCAGGTG |